Fig. 7. FILIA interacts with MATER. (A) Immunoprecipitation of MATER with antibodies to FILIA. Ovulated eggs (120) from wild-type (lanes 1,2,4) or Mater^tm/tm (lane 3) mice were lysed (0.5% Triton X100 in PBS) and either analyzed directly (lane 1) or immunoprecipitated with peptide-purified sheep antibodies to FILIA (lanes 2,3) or pre-immune sera (lane 4). Proteins were resolved by SDS-PAGE, transferred to nitrocellulose, incubated with peptide-purified rabbit antibodies to MATER and visualized with chemiluminescence. (B) Expression vectors containing full-length MATER (MYC-tagged) and FILIA were co-transfected into 293T cells (lanes 2,4). Controls (lanes 1,3) were co-transfected with the MYC vector lacking MATER and the FILIA expression vector. Whole cell lysates were analyzed before (left panel) or after (right panel) immunoprecipitation with antibodies to MYC. Proteins were resolved on SDS-PAGE gels, transferred to nitrocellulose and probed with antibodies to MATER (left) or FILIA (right panel). Molecular masses (kDa) are indicated at the left for both A and B.