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Figure 2


Fig. 2. Eomes expression in the NBL. (A-C) An E18 retina pulse-labeled with BrdU: anti-Eomes (A), anti-BrdU (B) and merged image (C). (D) At P0 in Math5+/- retinas, Eomes is expressed in the GCL and NBL. (E) At P0 in Math5-/- retinas, much of the Eomes expression is absent from the GCL but not the NBL. (F) At P12, some Eomes-positive cells in the GCL co-localize with NFL (arrowheads). Eomes is expressed in the nucleus and NFL is expressed in the cytoplasm. (G) At P30, Eomes is expressed in the GCL and in a subset of amacrine cells that are co-labeled with syntaxin (arrowheads). Syntaxin expression is cytoplasmic. The boxed region in the inset is enlarged to emphasize the co-localization of nuclear Eomes and cytoplasmic syntaxin expression. Scale bars: in C, 100 µm for A-C; in E, 100 µm for D,E; 10 µm in insets in F,G.