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Figure 5


Fig. 5. The expression of anterior neural markers and sprouty4 in the MZ hdf (fgfr1) medaka mutant. (A-D) Bud-stage (st. 18) embryos were hybridized with pax2, krox20 and ntl probes. (E-H) Twelve-somite-stage (st. 23) embryos were hybridized with bf1, pax2, krox20 and ntl probes. (I-P) Bud-stage (st. 18, I-L) and 12-somite-stage (st. 23, M-P) embryos were hybridized with a sprouty4 probe. The anterior is at the top. The genotypes of the embryos are indicated on the top of each column of panels. Note that the expression levels of bf1 at anterior telencephalon (black arrow in H), pax2 at MHB (red arrow in H) and sprouty4 at MHB (red arrow in P) are reduced in the MZ embryos at the 12-somite stage. AT, anterior telencephalon; M; maternal; MHB, midbrain-hindbrain boundary; MZ; maternal zygotic mutants; r3, rhombomere 3; r5, rhombomere 5; WT; wild type; Z, zygotic.