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Figure 2


Fig. 2. Forced Fz-LRP6 association activates LRP6 phosphorylation. (A,B) DKK1-Fz5, but neither Fz5 nor DKK3-Fz5, activated LRP6 phosphorylation (A) and TCF/β-catenin reporter expression (B) in HEK293T cells. The expression level of DKK1-Fz5 and DKK3-Fz5 was similar, but was much lower than that of Fz5 (A) as detected by an anti-Fz5 antibody. This was probably due to the presence of the DKK moiety. (C) A diagram showing the position of the amino acid substitutions or the truncation in DKK1-Fz5 mutants. (D) DKK1-Fz5, but neither of the DKK1-Fz5 mutants, enhanced LRP6 phosphorylation. The protein level of Fz5 and DKK-Fz5 fusion proteins was detected via the Myc tag. (E) DKK1-Fz5, but neither of the two DKK1-Fz5 mutants, synergized with LRP6 to stimulate the TCF/β-catenin reporter expression in HEK293T cells.