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Figure 4


Fig. 4. Fz recruitment of axin to the plasma membrane via Dvl (Xdsh) in Xenopus embryonic explants. Synthetic mRNAs for axin (0.5 ng), Xdsh (Dvl) or Xdsh{Delta}N or Xdsh-GFP (1 ng), and human Fz5 (1 ng) were injected alone or in combinations as indicated into the Xenopus embryo. Axin and Fz proteins were detected by anti-Flag and anti-Fz5 antibodies, respectively. (A) Axin alone. (Ba-c) Xdsh-GFP plus axin. (Ca-c) Fz5 plus axin. (Da-d) Fz5 plus axin plus Xdsh-GFP. (Ea-c) Fz5 plus axin plus Xdsh. (Fa-c) Fz5 plus axin plus Xdsh{Delta}N.