Fig. 7. AML1-ETO suppresses the monocytic cell fate. (A) In situ hybridization of l-plastin, mpo and pu.1. Tg(hsp:AML1-ETO) embryos were injected with gata1 morpholino (MO). Half of the injected embryos were heat-treated to induce AML1-ETO expression. AML1-ETO suppresses the expression of pu.1 and l-plastin but promotes the expression of mpo in gata1 morphants. (B) In situ hybridization of mpo. Injections of pu.1 MO decreased the expression of mpo in AML1-ETO-expressing embryos, inicating that Pu.1 is essential for the specification of granulocytes.