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Figure 1


Fig. 1. Identical phenotypes indicative of auxin starvation in DEX-induced expression of PIN7 or PGP19 in BY-2 cells. (A,B,D,E) Effect of DEX induction in GVG-PIN7 and GVG-PGP19-HA tobacco cell lines. Non-induced GVG-PIN7 (A) and GVG-PGP19-HA line (D). GVG-PIN7 (B) and GVG-PGP19-HA (E) cells after 3 days of cultivation with DEX, showing decrease in cell division, increase in cell elongation and formation of starch-containing amyloplasts (arrows in B,E). (C,F) Chemical inhibition of auxin transport (10 µM NPA) reversing these defects in DEX-induced GVG-PIN7 cells (C) but not in DEX-induced GVG-PGP19-HA cells (F). Scale bars: 20 µm. (G) Depiction (reciprocal plots) of the cell size distribution (cell length and cell diameter) after NPA treatment (10 µM, 3 days) in DEX-induced GVG-PIN7 and GVG-PGP19-HA cells scored at day 3 after inoculation. Non-induced GVG-PIN7 and GVG-PGP19-HA cells were used as a control.