Fig. 3. Expression and localization of PGP1 and PGP19 during Arabidopsis embryogenesis. (A,B) Immunolocalization of PGP1-myc in Arabidopsis embryos (PGP1 in red, DAPI in blue). Expression of PGP1-myc in all cells and non-polar localization to the plasma membrane at octant (o) (A) and mid-globular (mg) (B) stages. Inset shows staining in the suspensor (s). (C,D) PGP19-GFP localization during early embryogenesis. PGP19-GFP localizes apolarly to the plasma membrane in derivatives of the basal cells at the octant stage (C) and in the suspensor and lower tier cells at the dermatogens (d) stage (D). (E-J) Restriction of the expression of PGP19-GFP at later stages of embryogenesis to protoderm and cells surrounding the vascular primordium, which is mainly complementary to PIN1 expression. Immunolocalization at late-globular (lg) (E-G) and mid-heart (mh) stages (H-J) of PGP19-GFP (green) (E,H), PIN1 (red) (F,I). (G,J) Overlay of PIN1, PGP19 and DAPI (blue).