Fig. 6. Post-embryonic expression and the role of PGP1/PGP19 in lateral root development. (A,B) Expression and localization of PGP1-GFP and PGP19-GFP in root tips of 5-day-old seedlings. PGP1-GFP is expressed in all cells, except the columella (A); PGP19-GFP expression is more restricted to endodermal and pericycle cells (B). (C-F) Expression of PGP1-GFP and PGP19-GFP in hypocotyls and main root. PGP1-GFP is expressed in all cells of hypocotyls (C) and main root (D), whereas PGP19-GFP expression is more restricted to cells surrounding vascular tissues in hypocotyls (E) and main root (F). h, hypocotyl; r, root. (G,H) Expression of PGP1-GFP and PGP19-GFP during lateral root development. PGP1-GFP expression is detected in all cells during all stages (G) (indicated) and that of PGP19-GFP is more confined at later stages (indicated) to the new forming endodermal and pericycle cells (H). Arrowheads indicate the localization of PGP1/PGP19-GFP on anticlinal membranes at stage I. (I) Initiation and (J) emergence phenotypes of pgp1, pgp19, pin1, pgp1pgp19 and pin1pgp1pgp19 mutants (n=40).