Fig. 3. Neurod1 partially restores RGC axons and RGC subtypes in the absence of Math5. (A-D) Immunostaining of flat-mounted retinas from P30 mice with anti-NFL antibody to reveal RGC axons. Genotypes are indicated on the lower left of each panel. Insets in C and D highlight the misoriented axons. OD, optic disk. (E,F) Representative images of immunostaining of flat-mount retinas from P30 mice using anti-melanopsin antibody to reveal the mosaic distribution pattern of melanopsin-positive RGCs. The insets in E and F show representative dendritic arborization of melanopsin-positive RGCs in layer 1 of the IPL (arrowheads). Nuclei of the three nuclear layers are stained with DAPI (blue). Scale bar: 50 µm. (G,H) Immunostaining of flat-mount retinas with anti-SMI32 antibody to show the mosaic distribution of the SMI32-positive RGC subtype.