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Figure 6


Fig. 6. Loss of Cdk2 leads to premature transcriptional activation of Cdk1. (A,B) The β-galactosidase staining pattern in regenerating livers of Cdk2+/+ Cdk1+/Cdk2KI (Aa-d) and Cdk2-/- Cdk1+/Cdk2KI (Ba-d) mice at the indicated time points. (C,D) Immunohistochemical BrdU staining pattern in the regenerating livers of Cdk2+/+ (Ca,Da), Cdk2-/- (Cb,Db), Cdk2+/+ Cdk1+/Cdk2KI (Cc,Dc) and Cdk2-/- Cdk1+/Cdk2KI (Cd,Dd) mice at the indicated time points. (E) Bar chart showing the percentage of BrdU-positive liver cells at the indicated time points in regenerating livers of Cdk2+/+, Cdk2-/-, Cdk2+/+ Cdk1+/Cdk2KI and Cdk2-/- Cdk1+/Cdk2KI mice after 70% PH. The average and s.d. (error bars) were calcualted after counting a minimum of 3000 nuclei from several low-power fields per sample from each genotype. For each time point, four mice were analysed. Scale bar: 100 µm.