Fig. 7. Phenotypes of atp1b2b morphant fish (72 hpf). (A) Otic vesicles from wild-type embryos contained two otoliths (arrowheads). (B,C) atp1b2b morphant fish lacked one (B) or both (asterisk, C) otoliths and had ventral protuberances due to pericardial effusion (arrows), consistent with cardiac failure. (D) atp1b2b morphant neuromasts had significantly fewer hair cells than wild-type embryos. (E) Cardiac ventricular chamber sizes were smaller and contractile cycle length was longer (corresponding to slower heart rate) in atp1b2b morphant fish compared with wild-type fish. SSAx, systolic short axis; DSAx, diastolic short axis; CL, cycle length.