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Figure 2


Fig. 2. Immunostaining of Numb, cleaved Notch1 intracellular domain and active β-catenin in developing blast colonies. Immunostaining showing (A) Numb and activated β-catenin (β-cat), (B) cleaved Notch1 intracellular domain (NIC) and activated β-catenin (β-cat) in 12 hour, 24 hour and 48 hour blast colonies. Merged images show the overlay of three channels (DAPI, Cy2 and Cy3). Light transmission images (DIC) display the morphology of the cells. Arrows indicate the nuclear presence of activated β-catenin. Scale bars: 10 µm.