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Figure 8


Fig. 8. En1Cre/+; Otx2flox/flox and En1Cre/+; tOtx2ov/ov embryos exhibit complementary expression abnormalities in cell-cycle components. (A-L) Adjacent sections through the intermediate mesencephalon of E11.5 wild type, En1Cre/+; Otx2flox/flox and En1Cre/+; tOtx2ov/ov embryos pulsed with BrdU for 30 minutes and immunostained with BrdU and Nkx6.1 (A,C), Nkx2.2 and BrdU (B), Sox2 and p27kip1 (G-I) and BrdU and CycD1 (J-L) or hybridized with the Wnt1 probe (D-F).