Fig. 1. Structure of zou mutant alleles and ZOU expression. (A) Structure of ZOU genomic region and position of T DNA insertions. Exons are indicated by black rectangles; the direction of transcription is indicated by red arrow. The pSKI074 T-DNA insertion in zou1-D is shown as a triangle with black arrowheads indicating the viral enhancer sequences. The origin of the zou2-4 loss of function alleles is described in the Materials and methods. (B) RT-PCR analysis of gene expression in leaves of wild-type (+) and zou-1D/- (z) hemizygotes. Primer sets specific for three different genes were used, as indicated below the gel image. Eukaryotic translation initiation factor 4A (EiF4A) is a control for loading and RNA integrity. (C) Q-PCR analysis of ZOU expression in wild-type rosettes (R), seedlings (Se), unopened flower buds (B), opened flowers (F), siliques containing globular-heart stage embryos (S1) and siliques containing late heart and torpedo stage embryos (S2). Values are relative to expression in S1, which is designated as 1.0. No expression was detected in R, Se or F samples. Values are means of three replicates with standard error of mean.