Fig. 5. Expression of diverse genes involved in endosperm and embryo development in zou mutant seeds. (A-D) Images of in situ hybridization of digoxigenin-labelled ALE1 antisense RNA probes to sections of siliques, viewed using DIC microscopy. Embryo is indicated by arrowhead, endosperm by arrows. Little or no ALE1 expression was detected in zou-2 mutant seeds (C,D), whereas wild type showed intense signal in ESR (A,B). (E,F) Confocal images of reporter line 9185 in wild-type (E) and zou-4 (F) seeds. (G,H) Images of reporter line ATML1::GFP-ATML1 in wild-type (G) and zou-4 (H) seeds. (I,J) Images of reporter line ACR4::H2B-YFP in wild-type (I) and zou-4 (J) seeds. Scale bar: 100 µm.