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Figure 2


Fig. 2. Shh promotes neurite outgrowth from VT explants but inhibits neurite outgrowth from DN explants. (A) Ventrotemporal (VT) and dorsonasal (DN) explants grown in the presence of different Shh concentrations. Outgrowth was visualised by staining with phalloidin. (B,C) Quantification of neurite outgrowth from DN (B) and VT (C) explants. Bar graphs show the area covered by neurites (in pixels). The number of explants analysed is indicated above each bar. *P<0.05, **P<0.01 (one-way ANOVA). Scale bar: 500 µm.