Fig. 2. Conserved non-coding elements in the Hoxb5 genes of vertebrates. Sequences from mouse, human, zebrafish and Takifugu were compared using Pipmaker. The mouse Hoxb5 locus is used as a reference and is shown on the horizontal axis. Sequences from human (Hs), zebrafish (Dr) and Takifugu rubripes (Tr) are compared and the similarity is shown on the vertical axis. CNE positions are indicated by shading: red, J1; blue, J2; green, J3. A sequence similarity lower than 50% was observed between the J2 elements from Takifugu hoxb5a and mouse Hoxb5 using a different algorithm. Identical results to those shown were obtained when teleost sequences were used as a reference. The annotations at the top refer to the mouse reference sequence: exons (black boxes), simple repeats (vertical rectangles) and CpG islands (white and gray rectangles).