Fig. 9. Phylogenetic analysis of Hoxb5 loci from human, mouse, zebrafish and Takifugu. (A) Phylogenetic tree predicted by the DDC model. Its topology is based on the assumption that hoxba and hoxbb teleost complexes resulted from a duplication event that happened after the separation of the fish and tetrapod lineages and before the divergence of Takifugu and zebrafish lineages. (B-F) Phylogenetic trees built with MEGA3 based on the following sequences: (B) Hoxb5 coding sequences (850 bp); (C) 5' upstream region of Hoxb5 genes (130 bp); (D) J1 CNE (255 bp); (E) J2 CNE (150 bp); (F) CNEs present in all Hoxb5 loci (535 bp), for which the 5' upstream region, J1 and J2 were joined in a continuous single sequence for each Hoxb5 locus. As the J3 CNE was only found in the hoxb5a complexes of zebrafish and Takifugu, it was not included in this analysis. Hs, human; Mm, mouse; Dr, zebrafish; Tr, Takifugu. The scale bar indicates an estimated evolutionary distance of 0.05 nucleotide substitutions per site. Numbers at nodes indicate the bootstrap values.