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Figure 4


Fig. 4. Neuronal and glial differentiation of ENCCs. Immunohistochemical analysis of control (A-C,G) and Pofut1 cKO (D-F,H) mouse embryos with anti-TuJ1 (red) and anti-GFP (green) antibodies to visualize neuronal cells and ENCCs, respectively, at E10.5-12.5 (A-F), or with anti-BFABP (magenta) and anti-GFP (green) antibodies to visualize glial progenitors and ENCCs, respectively, at E11.5 (G,H). Arrowheads (G,H) indicate BFABP-positive ENCCs. (I,J) Quantitation of neuronal differentiation at E10.5 (I) and E11.5 (J). Average percentages of TuJ1-positive ENCCs are shown with s.d. (K) Quantitation of glial differentiation at E11.5. Average percentages of BFABP-positive ENCCs are shown with s.d.