Fig. 7. ENCCs expressing Hes1 are decreased, whereas those expressing Mash1 are increased, in the Pofut1 cKO embryo. Immunohistological analysis of control (A-C,G-I) and Pofut1 cKO (D-F,J-L) mouse embryos at E10.5 using antibodies against Hes1 (A,C,D,E, magenta), Mash1 (G,I,J,L, magenta) and GFP (B,C,E,F,H,I,K,L, green). Arrowheads indicate Hes1-positive or Mash1-positive ENCCs in control (C,I) and Pofut1 cKO (F,L) embryos. (M,N) Quantification of Hes1-positive ENCCs (M) and Mash1-positive ENCCs (N) at E10.5. Average percentages of the total ENCC cell population expressing each gene are shown with s.d.