Fig. 9. An increase in the number of astrocytes in the Fz5^-/- retina. (A,B) Flat-mount mouse retinas at P5 immunostained for Gfap to visualize astrocytes. The optic disc is at the lower left in all panels (asterisk). (C-H) Flat-mount retinas at P42 showing astrocytes (Gfap) and the vasculature (lectin IB4) on the inner face of the retina. Merged images from C-F are shown in G,H. At P42, the retinal vasculature appears normal. At both ages (P5 and P42), the Fz5^-/- retina has an increased density of astrocytes compared with the WT.