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Figure 7


Fig. 7. R-spondin signaling promotes angiogenesis in vitro. (A-C) Chicken chorioallantoic membrane assay using beads soaked with Rspo2 or VEGF or control. (D) Cell proliferation assay using BrdU incorporation in human umbilical vein endothelial cells (HUVEC). RLU, relative light units. (E) Cell proliferation assay in control (wt) and Rspo3-/- (null) E9.5 embryos using 4 hours BrdU incorporation in vitro (n=9 embryos each). (F,G) Induction of capillary-like morphology of human dermal microvascular endothelial cells after treatment with recombinant Rspo2. (H-J) Three-dimensional spheroid capillary sprouting assays using HUVEC. (K,L) Quantification of spheroid assays. CSL, cumulative sprout length.