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Figure 6


Fig. 6. S458 phosphorylation in embryos and oocytes. (A-F') Each row shows a single one-cell stage wild-type or mutant embryo, as listed after staining with {alpha}MEX-5 (left column) and {alpha}MEX-5(pS458) (right column). (G-I') Each row shows one arm of a wild-type or mutant gonad, as listed after staining with {alpha}MEX-5 (left column) and {alpha}MEX-5(pS458) (right column); gonads are oriented as in Fig. 1B. {alpha}MEX-5(pS458) shows non-specific staining of cytoplasmic foci in early embryos (B',D',E',F') and of sperm (sp) in gonads (see H'). par-1(it51), but not par-1(b274), has a mutation in the predicted kinase domain.