Fig. 2. endos⁰⁰⁰⁰³ mutants have spindle defects. Stage 14 oocytes (A-F) and 0- to 3-hour embryos (G-O) stained with propidium iodide (DNA, red) and anti--tubulin (microtubules, green). (A) Control oocytes in metaphase I. (B) Typical endos⁰⁰⁰⁰³ oocyte showing dispersed DNA without a spindle. (C) Rare endos⁰⁰⁰⁰³ oocyte showing abnormal DNA associated with large spindle masses. (D,E) Similar twine¹ phenotypes. (F) elgi¹ oocyte in metaphase I. (G) Control embryos exhibiting typical embryonic mitoses. (H-J) endos⁰⁰⁰⁰³-derived embryos showing dispersed DNA with no spindle (H), dispersed DNA with spindle masses (I), and tripolar spindles (J). twine¹-derived embryos showing dispersed DNA (K), abnormal spindle masses (L), spindle asters with no DNA (M), and long, thin spindles (N). (O) Embryos derived from elgi¹ females showing normal spindles and DNA. Scale bars: 10 µm.