Fig. 2. The GATA4-FOG2 complex is essential for the activation of
dimorphically expressed genes. (A-G,J-M) Whole-mount ISH was
performed with Sprr2d (A-D), Foxl2 (E-G), Gng13
(J,K) and Sf1 (Nr5a1; L,M) RNA probes on XX gonads from
wild-type, Gata4ki/ki and Fog2-/-
E12.5-13.5 mouse embryos as indicated. (H,I) Immunofluorescent
staining of frozen gonadal sections from Fog2+/- (control)
or Fog2-/- embryos with an anti-FOXL2 antibody (red).
Embryonic germ cells are detected by the anti-PECAM1 antibody (e.g.
Yao et al., 2003) (green).
Magnification: 200x.