Fig. 3. GATA4/FOG2 loss affects multiple aspects of early ovarian differentiation without activating Sertoli cell differentiation. (A,B) Whole-mount ISH was performed on XX E12.5 control or Gata4^ki/ki gonads (A) or XY E13.5 control or Fog2^-/- gonads (B) with the indicated probes. Note that the expression of Sertoli cell markers (Sox9, Mis and Dhh) is not increased in E12.5-13.5 XX gonads upon GATA4/FOG2 loss, whereas expression of genes encoding steroidogenic enzymes (e.g. Hsd3b1) is relaxed. Scale bar: 1 mm. (C) ISH was performed with an Inha probe on XX and XY wild-type and Fog2^-/- gonads. Expression of Inha is derepressed in the XX Fog2^-/- gonad. (D) The coelomic blood vessel does not form in Fog2^-/- gonads. XX and XY gonads from E12.5 mouse embryos carrying Flk1-lacZ with wild-type or homozygous mutant Fog2 were stained with X-Gal, which marks the Flk1-lacZ-positive endothelial cells. The coelomic blood vessel in the testis is indicated by arrows.