Fig. 7. GATA4/FOG2 loss does not affect the initiation of germ cell sexual differentiation. (A) ISH was performed with an Oct4 RNA probe on XX gonads from wild-type or Fog2^-/- mouse embryos as indicated. (B-D) Immunofluorescent staining of frozen sections with an anti-H2AX antibody (red). Embryonic germ cells are detected by the anti-PECAM1 antibody. Note the anti-H2AX staining in the normal ovaries (C) and mutant gonads (D), but not in the control testis (B). (E) qRT-PCR analysis of Stra8 and Scp1 gene expression in wild-type and Fog2^-/- E13.5-14.5 XX and XY gonads. The y-axis shows values for both genes normalized to Gapdh RNA copy number.