Fig. 1. sktl affects axis formation in the Drosophila oocyte. (A,B) Egg chambers expressing GFP-Stau. (A) Wild type (WT). (B) l(2)2.3 mutant germline clone. (C,D) Dorsal view of an egg shell. (C) In WT, the two appendages mark the dorsal side of the egg. (D) In the l(2)2.3 mutant germline clone, the egg is ventralized and lacks dorsal appendages, but the posterior aeropyle is normal (compare insets in C and D). (E,F) Homozygous l(2)2.3 mutant clones, marked by the absence of GFP (green). Stau protein localization (red) and nucleus positioning are not affected when the posterior follicle cells are mutant (E), but are lost in mutant germline clones (F). (G,H) sktl mRNA expression. The level of sktl mRNA (blue) is reduced in l(2)2.3 mutant (H) as compared with WT (G) nurse cells. Anterior is to the left and posterior is to the right. Asterisk, nucleus.