Fig. 5. Sktl is required to properly organize the cortical domains of the oocyte. (A) Baz-GFP localizes strongly at the anterolateral cortex of the WT Drosophila oocyte. (B) In sktl^2.3/sktl⁵ oocytes, Baz-GFP has lost most of its cortical localization and is found as puncta in the cytoplasm. (C,D) skltl^M5 follicle cell clone (FCC; dashed line) marked by the absence of GFP (green) and stained for Baz (red in C, white in D). (E,F) Cortical localization of Lkb1 (E) is maintained in sktl^2.3/sktl⁵ oocytes (F). (G) Baz-GFP^S151A,S1085A overexpression induces oocyte polarity defect phenotypes, with nucleus mispositioning and ventralized eggs (inset in G). (H) Lgl-GFP is localized at the posterior of the oocyte. (I) In sktl^2.3/sktl⁵ oocytes, Lgl-GFP is no longer enriched at the posterior and is instead detected mainly in the cytoplasm and along the cortex. (J) GFP-Par-1 (N1S) is enriched at the posterior at stage 9 (arrowhead). (K) In sktl^2.3/sktl⁵ oocytes, GFP-Par-1 (N1S) is no longer enriched at the posterior and instead has started to accumulate all along the cortex and in the cytoplasm. Asterisk, nucleus.