Fig. 7. Sktl activity is required for Moesin phosphorylation. (A,B) Immunostaining using an antibody specific for the phosphorylated form of Moesin (P-Moe). P-Moe staining is found at the cortex in WT Drosophila oocytes (red in A) and is abolished in sktl^2.3 mutant germline clones marked by the loss of GFP (B). (C,D) Western blot of ovary extracts (1, 2 and 4 mean that the equivalent of 1, 2 and 4 ovaries were loaded) from WT and sktl^2.3/sktl⁵ probed with anti-P-Moe (C), anti-Moesin (D) and anti-Tubulin (-tub) as a loading control (C,D). (E,F) Immunostaining using an antibody against Moesin. Moesin is localized at the cortex in both WT and sktl^2.3/sktl⁵ oocytes (E,F, arrowheads). Asterisk, nucleus.