Fig. 2. Cleft palate phenotype in Wnt5a mutant. (A) An E18.5 wild-type mouse shows an intact palate. (B) An E18.5 Wnt5a mutant shows wide open cleft secondary palate. (C,E) Coronal sections of an E13.5 control embryo show the normal palatal shelves at the anterior (C) and posterior domain (E). Arrows in C indicate the ventrolateral indention. (D) A coronal section of an E13.5 Wnt5a mutant shows the palatal shelves in the horizontal level lacking the ventrolateral indention (arrows). (F) The posterior palatal shelves of an E13.5 mutant exhibit an abnormal shape. (G,I) An E14.5 control embryo shows closure of the palate at the anterior (G) and posterior region (I). (H) The anterior palatal shelves of an E14.5 Wnt5a mutant remain at the horizontal level but appear too short to make contact at the midline. (J) The posterior palate of an E14.5 mutant fails to elevate, remaining at the vertical position. T, tongue; PS, palatal shelf.