Fig. 6. Regulation of cell proliferation in palatal mesenchyme by Wnt5a is mediated by Ror2. (A) An explanted wild-type palatal shelf implanted with BSA bead shows BrdU-labeled cells in the distal (the MEE side) mesenchyme. (B) An explanted wild-type palatal shelf implanted with a Wnt5a-soaked protein shows a reduced rate of cell proliferation in the distal mesenchyme. (C) A higher cell proliferation rate is seen in an explanted Wnt5a^-/- palatal shelf implanted with a BSA bead. (D) Cell proliferation rate is rescued to about the normal level in an explanted Wnt5a^-/- palate by a Wnt5a-soaked bead. (E,F) Elevated cell proliferation rate is seen in an explanted Ror2^-/- palatal shelf implanted with a BSA bead (E), and remains in an explanted Ror2^-/- palatal shelf implanted with a Wnt5a bead (F). (G) Comparison of percentage of BrdU-labeled cells in the fixed area (box) of the palate of different genotypes implanted with BSA- or Wnt5a-soaked beads. Standard deviation values were indicated as the error bars. B, bead. In all images, the MEE is towards the left. ^*P<0.01.