Fig. 7. Lack of Foxp2 expression in the postnatal Dicer-ablated cortex. (A-D) Immunofluorescence confocal microscopy of 10 µm coronal cryosections (A,B; 3-µm single optical sections) or 50 µm coronal Vibratome-produced sections (C,D; 6-µm single optical sections) through the cerebral cortex of control (Emx1^Cre/wt Dicer^flox/wt; A,C) and conditional Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox; B,D) P0 (A,B) and P7 (C,D) littermate mice, showing DAPI (blue) and Foxp2 (red) staining. Note the almost complete absence of Foxp2-positive neurons in the cerebral cortex of P1 and P7 Dicer-ablated mice. Ventricular surface (VS), layers VI and V-I, and the pial surface (PS) are indicated on the left. Scale bars: 50 µm in A,B; 100 µm in C,D.