Fig. 7. Expression of Oct4, a pluripotency marker, and repression of nuclear lamin A (LMNA), a tissue-specific marker, in cells treated with GV cytoplasmic lysate and cultured in DMEM or ES medium. B6D2F1 strain mouse fibroblasts were treated with GV cytoplasmic lysate and cultured for 0, 1, 2, 3 or 4 weeks in DMEM with 10% FBS (DMEM medium) or in medium designed for embryonic stem cell culture (ES medium). (A) PCR analysis of Oct4 and LMNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a normalization control. Expression of Oct4 and repression of LMNA is clearly shown after 4 weeks of culture in both DMEM and ES medium. (B,C) Quantitative RT-PCR analysis of Oct4 (B) and LMNA (C). SC, somatic cell; ES, embryonic stem cell. Asterisks indicate the significant differences between expression of Oct4 and repression of LMNA at 4 weeks of culture.