Fig. 10. Transcriptional regulation of Tbx22 by Mn1. (A) Tbx22 promoter-luciferase reporter activity in transfected NIH3T3 cells in the absence or presence of increasing amounts of co-transfected Mn1 expression vector. Luciferase activity was normalized against the control (promoter-less pGL3-basic) vector-transfected cells. Error bar represents standard deviation. hP0, hP0 promoter; hP1, hP1 promoter; mP0, mouse Tbx22 P0 promoter; mP1, mouse Tbx22 P1 promoter. (B,C) Effect of Mn1 overexpression on endogenous Tbx22 mRNA expression in NIH3T3 cells. Expression levels of Mn1 (B) and Tbx22 (C) mRNAs were quantified by real-time RT-PCR and normalized against mock-transfected cells. Error bar represents standard deviation.