Fig. 7. Expression of cyclin D2 was downregulated in Mn1^-/- mutant palatal shelves at E13.5. (A,D) In the anterior region of the developing palate, expression of cyclin D2 was similarly weak in wild-type (A) and Mn1^-/- mutant (D) embryos. (B,E) In the middle palate, cyclin D2 is highly expressed in both the epithelium and mesenchyme in the wild-type embryo (B) but its expression is much reduced in the Mn1^-/- palatal mesenchyme (E). (C,F) In the posterior palate region, strong cyclin D2 expression was detected in both the epithelium and mesenchyme in the wild-type palate (C) but was much reduced in the Mn1^-/- mutant embryo (F). Green arrows in C and F indicate the medial edge epithelium of the palatal shelves.