Fig. 2. miR-126^/, but not Egfl7^/, mice exhibit incompletely penetrant embryonic lethality, edema and vascular leakage. (A,B) Breeding tables from heterozygous intercrosses show that Egfl7^/ mice are born at normal Mendelian ratios (²=0.741), whereas miR-126^/ embryos exhibit 50% embryonic/perinatal lethality (²<0.001). Numbers in parenthesis indicate embryos found with edema. Red numbers indicate deviation from Mendelian ratios. (C) Wild-type (wt) and Egfl7^/ embryos were phenotypically indistinguishable, whereas 50% of the miR-126^/ embryos displayed subcutaneous edema (^*) at E14.5. (D) Hematoxylin and Eosin staining reveals the severity of the edema (^*) in E14.5 embryos. (E) Varying degrees of subcutaneous hemorrhage are detected in 20% of E15.5 miR-126^/ embryos; a severely affected embryo is depicted. Histological analysis shows red blood cells extravasating from a representative ruptured, leaky vessel (arrow). (F) Tie2-Cre-mediated endothelial deletion of miR-126 phenocopies the miR-126^/ edema (^*) phenotype.