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Figure 3


Fig. 3. Angiogenesis phenotypes in miR-126{Delta}/{Delta} embryos. (A) Isolectin B4 staining of P5 postnatal retinas. Retinal vascularization was normal in Egfl7{Delta}/{Delta} mice but was severely delayed in miR-126{Delta}/{Delta} mice as indicated by arrows. The dashed line indicates the edge of the optic cup. wt, wild type. (B) Quantitation of retinal vascularization demonstrates a ~40% reduction of retinal vascular coverage in miR-126{Delta}/{Delta} mice. (C) High-magnification images of retinal vascular sprouts. Note the marked thickening (arrows) of vascular sprouts in miR-126{Delta}/{Delta} retinas as compared with wild-type or Egfl7{Delta}/{Delta} mice. (D) CD31 (Pecam1) whole-mount staining of E12.5 heads. Note the delayed vascularization and reduced complexity (arrow) of the cranial vasculature in miR-126{Delta}/{Delta} embryos. (E) Adult animals of the indicated genotypes (n=7) received corneal implants of slow-release hydron pellets containing VEGF. Neovascularization was quantified after 6 days by slit lamp examination. Arrows highlight the length of vessel growth. (F) Adult miR-126{Delta}/{Delta} mice exhibited ~50% impairment of corneal vascularization relative to Egfl7{Delta}/{Delta} or wild-type mice.