Fig. 1. PTK7 recruits dsh to the plasma membrane. Animal caps were injected with different tagged RNAs, and protein localization was analyzed by confocal microscopy. The GFP-tagged dsh (green, left), the co-expressed myc-tagged protein (red, middle) and the merged pictures (right) are shown. (A) GFP-tagged dsh is localized to the cytoplasm of animal caps injected with 100 pg dsh-GFP RNA. (B) Myc-tagged fz7 is predominantly membrane localized in animal caps injected with 100 pg fz7-myc RNA. (C) Co-injection of 100 pg dsh-GFP and 100 pg fz7-myc RNA leads to membrane recruitment of dsh. (D) PTK7 is membrane localized in animal caps injected with 500 pg myc-tagged PTK7 RNA. (E) Animal caps co-injected with 100 pg dsh-GFP RNA and 500 pg PTK7-myc RNA show membrane-recruitment of dsh. Cells that do not express PTK7 in the membrane do not show membrane-localization of dsh (white arrowhead). (F) Animal caps injected with 250 pg RNA coding for a PTK7 mutant lacking the conserved kinase domain (kPTK7) and 100 pg dsh-GFP RNA do not show membrane localization of dsh. (G) Graph summarizing the percentage of cells with membrane-localized dsh. For colocalization assays using PTK7 or fz7, only cells in which these proteins were membrane localized were analyzed. To determine the number of cells with cytoplasmic dsh localization DAPI co-staining was used. The total number of cells is indicated above each column.