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Figure 2


Fig. 2. The PDZ domain is necessary for PTK7-dependent membrane translocation of dsh. (A) Dsh protein structure indicating the DIX, PDZ and DEP domain. RNA of GFP-tagged deletion mutants of these domains were expressed alone or in combination with wild-type PTK7 RNA in animal caps. Protein localization was determined by confocal microscopy. (B) Animal cap injected with 100 pg {Delta}DIX-GFP RNA showing cytoplasmic protein localization. (C) Co-injection of 500 pg PTK7-myc RNA partially translocates the cytoplasmic {Delta}DIX-GFP protein to the membrane. (D) Animal caps injected with 100 pg {Delta}PDZ-GFP RNA show only cytoplasmic localization of the protein. (E) The same is seen after co-expression with PTK7-myc RNA. (F) Animal caps injected with 100 pg {Delta}DEP-GFP RNA express the protein in the cytoplasm, whereas (G) co-injection of 100 pg PTK7-myc RNA leads to membrane recruitment. (H) Graph summarizing the percentage of cells with membrane-localized dsh. Only cells with membrane expression of PTK7 were analyzed for dsh localization. The total number of cells is indicated on each column.