Fig. 1. Differential response to Pax6 loss in subpopulations of Pax6^lacZ/lacZ OV progenitors. (A-L) Crx expression was characterized by fluorescent in situ hybridization (A-D,I-L, green), the distribution of VC1.1 epitope (E-H, green K,L; red) and Pax6 (A-H, red) was monitored by antibody labeling, in the control Pax6^+/+ (A-H) and Pax6^lacZ/lacZ (I-L) embryos. The insets in A,E,I,C,G,K are enlarged in B,F,J,D,H,L. inl, inner nuclear layer; le, lens; nr, neuroretina; oc, optic cup; os, optic stalk; prp, prospective photoreceptor layer; rpe, retinal pigmented epithelium. Scale bars: in A, 50 µm for A,E,I,C,G,K; in B, 10 µm for B,F,J,D,H,L.