Fig. 2. Zic2 is expressed ectopically in nasal retina of Foxg1^-/- embryos. Zic2 (green) and Brn3a (red) immunohistochemistry in (A-H) wild-type and (I-P) Foxg1^-/- retinas at E14.5. (E-H) Higher magnifications of boxed areas in A-D, respectively. (M-P) Higher magnifications of boxed areas in I-L, respectively. (A,B,E,F) In wild-type dorsal retina, there are few Zic2-expressing cells in the inner retinal (RGC) layer; some are seen in other layers and there is strong expression in CMZ (marked in F), as reported previously (Herrera et al., 2003). (C,D,G,H), Zic2-expressing inner retinal layer cells are found predominantly in VT retina clustered adjacent to the CMZ (in boxed area in C). (I,J,M,N) In Foxg1^-/- dorsal retina; numerous Zic2-expressing cells are seen nasally, adjacent to the CMZ in the inner retinal layer (boxed area in J). (K,L,O,P) In Foxg1^-/- ventral retina; Zic2-expressing inner retinal layer cells are seen both nasally and temporally (boxed areas in K and L). Abbreviations: N, nasal; T, temporal; cmz, ciliary marginal zone. Scale bars: 200 µm in A-D,I-L; 100 µm in E-H,M-P.