Fig. 5. Foxg1 is not required for retinal outgrowth in culture. (A) DN Foxg1^+/±, (B) VT Foxg1^+/±, (C) DN Foxg1^-/- and (D) VT Foxg1^-/- retinal explants cultured in collagen without chiasm cells. (A-D) Neurofilament (NF) immunohistochemistry revealed neurite outgrowth from all explants. (A'-D') Brn3a expression in co-cultures A-D respectively revealed healthy RGCs. (E) Mean percentage axon coverage (±s.e.m.) for Foxg1^+/± and Foxg1^-/- retinal explants from DN and VT retina 45 µm from the retinal explant (measured as in Fig. S2 in the supplementary material). (F) Mean length of the longest neurites (±s.e.m.) for each set of explants. (E,F) Numbers of explants are indicated in parentheses. (E) One-way ANOVA showed a significant effect of explant type; significant differences are marked by brackets with P values indicated. (F) One-way ANOVA revealed no significant differences in the lengths of outgrowth among retinal explants. Abbreviations: DN, dorsonasal; VT, ventrotemporal. Scale bars: 100 µm in A-D; 10 µm in A'-D'.