Fig. 7. Loss of Foxg1 from retina or chiasm has no significant effect on outgrowth of ventrotemporal retinal axons on chiasm cells in culture. (A-D) Co-cultures of Foxg1^+/± or Foxg1^-/- ventrotemporal (VT) retinal explants with dissociated Foxg1^+/± or Foxg1^-/- chiasm cells; immunohistochemistry and counterstaining are as in Fig. 6. (A'-D') Brn3a expression in co-cultures A-D, respectively. (E,F) Mean percentage of axon coverage (±s.e.m.) 45 µm from the retinal explant (E) and mean lengths of the five longest neurites (±s.e.m.) (F). One-way ANOVA revealed no significant differences in outgrowth among retinal explants. (E,F) Numbers of explants are in parentheses. Scale bars: 100 µm in A-D; 10 µm in A'-D'.