Fig. 8. Morpholino against Slc6a20a suppresses betaine transport in mouse parthenogenotes. (A) Effect of morpholinos on betaine transport in mouse parthenogenotes. Rates of betaine transport were determined after injection of MOs into GV oocytes followed by in vitro maturation and parthenogenetic activation with Sr²⁺. The MO designed to block translation of SIT1 (Slc6a20a) significantly decreased the rate of betaine transport (a versus b, P<0.01 by ANOVA and Tukey-Kramer post-hoc test) relative to uninjected parthenogenotes (none) or those injected with a control MO. Each bar represents the mean±s.e.m. of three independent experiments, each containing 8-12 oocytes. (B) Effect of MOs on functional expression of exogenous mouse SIT1 in Xenopus oocytes. Xenopus oocytes were co-injected with Slc6a20a cRNA and MOs (labeled as in A). Induced proline uptake was significantly decreased by the Slc6a20a MO (a versus b, P<0.001 by ANOVA and Tukey-Kramer post-hoc test). Each bar represents the mean±s.e.m. proline uptake in seven to eight individual oocytes after subtraction of the mean uptake in water-injected controls.