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Figure 3


Fig. 3. Binding site diagram and sequence of eve PRE300. The line diagram at the top and the sequence below show the locations of consensus binding sites for the known PRE-binding proteins Dsp1 (red), Zeste (blue), Sp1 (green) (Brown et al., 2005), Grh (purple) (Blastyak et al., 2006), and the functional Pho- (black, bold) and GAF-binding sites (black, solid underlines) based on our mutational analysis (see text). PRE300{Delta}gaga1 (see Fig. 1) has the 5'-most GAF site mutated, whereas PRE300{Delta}gaga12 has the 5'-most and the 3'-most sites mutated. The extents of the sub-elements PRE300-5' and PRE300-3' are shown as solid lines in the diagram. The sequences used to eliminate the GAF and Pho sites are shown under the main sequence. BsrGI and EcoRI restriction enzyme sites are marked with broken underlines.