Fig. 2. Pcdh- proteins localize to retinal plexiform layers during postnatal development. (A-D) Immunolabeling of Pcdh- ^fusg/fusg retina at P0 (A), P3 (B), P7 (C) and P14 (D) with GFP antibody. At P0 (A) and P3 (B), Pcdh--GFP proteins are present in the IPL and retinal axon layer (arrow), and are distributed around cell somata in the neuroblast layer (NBL; other abbreviations as in Fig. 1). At P3, Pcdh- proteins are also present in presumptive horizontal cells (asterisk; inset in B). (C) By P7, Pcdh--GFPs are detected in the emerging OPL, as it develops between the ONL and INL. (D) By P14, the adult pattern of Pcdh--GFP localization (see Fig. 1) is attained. Scale bars: 20 µm in A; 10 µm in inset.