Fig. 2 . Follicle-cell fate markers are unaffected in lgl germline clones. (A-H') Cut is an immature cell fate marker that is downregulated by Notch signaling at stage 6. Hindsight (Hnt) is expressed after stage 6 and Eyes absent (Eya) is normally expressed in anterior follicle cells and immature follicle cells. Pointed-lacZ is a posterior follicle-cell fate marker. No differences in expression of these markers between lgl⁴ germline clones (B,D,F,H) and the wild type (A,C,E,G) were detected. Mutant germline clones were marked by the absence of GFP (green) in the nuclei of the nurse cells.