Fig. 5. Reduced size of secondary sympathetic ganglia in Insm1 mutant mice. Secondary sympathetic ganglia of Insm1^lacZ/+ and Insm1^lacZ/Insm1^lacZ mice (E18.5) were analyzed by (A,B) X-gal staining and by (C-H) immunohistochemistry. Antibodies used were directed against: (C,D) Tuj1 (red) and Th (green), (E,F) Tuj1 (red) and NPY (green); (G,H) Phox2a (red) and Tlx3 (green). Arrowheads in A and B indicate superior cervical and stellate ganglia. Scale bars: 500 µm in A; 100 µm in C.